To unlock an Ascendancy, you must complete a series of trap gauntlets named the Trials of Ascendancy. There are twelve you can find in the campaign, the first six being required to earn your prestige class. When the first six Trials are complete, interact with the statue in Act 3 to open the Aspirant's Trial, allowing you to enter the Lord's Labyrinth. Successfully completing a Labyrinth run without dying unlocks your Ascendancy.
There are four variants of Labyrinth that each grant Ascendancy points when cleared for the first time. The first six Trials unlock Normal Labyrinth, the next three unlock Cruel, and the final three unlock Merciless Labyrinth. A fourth difficulty is also available once you reach Path of Exile's endgame, giving you a total of eight Ascendancy points to spend.
For a list of Ascendancies and their respective nodes, consult this Path of Exile webpage.
What is likely more useful for you are the locations for each Trial. They are listed below in order of appearance:
Close
Normal Labyrinth
Act
Zone
Act 1
The Lower Prison
Act 2
The Crypt (Level 1)
Act 2
The Chamber of Sins (Level 2)
Act 3
The Crematorium
Act 3
The Catacombs
Act 3
The Imperial Gardens
Cruel Labyrinth
Act 6
The Prison
Act 7
The Crypt
Act 7
The Chamber of Sins (Level 2)
Merciless Labyrinth
Act 8
The Bath House
Act 9
The Tunnel
Act 10
The Ossuary
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Additionally, players can complete the Eternal Labyrinth once they reach Maps, Path of Exile's endgame. Completing a Labyrinth Trial that randomly spawns in a Map will grant an Offering to the Goddess, a currency type required to start an Eternal Labyrinth run. Finish the run without dying to receive your final two Ascendancy points.
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